Academic management

University of Oviedo

Uniovi.es | Home | Search | Site Map

| |

Master's Degree in Biotechnology of Environment and Health
MBMAMS01-1-004
Genomic and Nucleic Acid Biotechnology
General description and schedule Teaching Guide

Coordinator/s:

JOSE FRANCISCO PARRA FERNANDEZ
fparrauniovi.es

Faculty:

Gonzalo Machado-Schiaffino Ferrer
machadogonzalouniovi.es
(English Group)
EDUARDO CIRES RODRIGUEZ
cireseduardouniovi.es
(English Group)
Ángel Luis Alvarez Rodríguez
alvarezrangeluniovi.es
(English Group)

Contextualization:

Genomic and Nucleic Acid Biotechnology is one of the compulsory subjects in the Basic module, which is taught in the first semester of the first year of the Master in Biotechnology of Environment and Health.

Requirements:

To follow the course it is desirable to have knowledge of genetics and molecular biology at undergraduate level.

Competences and learning results:

To provide expertise in the major methodologies and systems used in biotechnology, with special emphasis on applications to health and the environment. The main expected learning outcomes are: 

  • Proper preparation, discussion and presentation by the student of tasks provided.
  • To be able to select and design bioinformatic analytical methodologies and appropriate cellular experimental systems for particular biotechnological purposes.
  • To carry out safely experimental activity in the laboratory.
  • To develop the capacity to propose new strategies and improved techniques for the production of molecules of industrial interest in food, medical, pharmaceutical or other biotechnological applications, based on a practical knowledge of genomics and an understanding of the mechanisms of gene expression.

Contents:

Structural genomics

  • Genes and genomes: structure and evolution
  • DNA sequencing methodologies
  • Molecular markers and its applications
  • Genetic and physical mapping

Bioinformatic tools for genetic analyses

  • Molecular Sequences and Structures
  • Biological Databases
  • Basic Local Alignment Search Tools (BLAST)
  • Sequence Alignment
  • Molecular Phylogenetics
  • Eukaryotic Genomic Databases: Plant Genomes

Gene function analysis

  • cDNA libraries
  • post-transcriptional gene silencing and RNA interference
  • CRISPR/Cas9
  • Lentiviral vectors, CRISPR libraries and RNAi libraries

 

Methodology and work plan:

In-person activities may be carried out online if necessary.

The methodology will consist of blocks with the following sequence of activities: 

1 – One-hour lecture (classroom activity): it consists of a presentation by the teacher of the main objectives of the subject, developing the most relevant topics or those posing specific difficulties for students. The teacher will propose practical, realistic problems related to the contents and objectives of the subject matter, to be resolved by students. 

2 –Eight to nine hours of student’s personal work: based on the above lecture contents, students will prepare a seminar presentation about a study case, or they will carry a bibliographic research, or propose solutions to a practical case raised by the teacher. This will serve to gain further insight into the topic covered by lectures, to achieve a better understanding of the subject and its implications, or to apply learned concepts to real situations. 

3 – Two hours of student seminars or presentations: students will present and discuss under the supervision of the teacher, the result of their personal work mentioned in section 2. These two hours of classroom activities will take place far enough after the corresponding introductory lecture, so that students will have adequate time to do the necessary personal work. 

4 – An average of half an hour of group-tutoring for each of the previous blocks, usually at some point during the implementation of paragraph 2. 

The course consists essentially of 8 blocks of this type. These blocks may be consecutive to each other, cyclically, or may partially or completely overlap, so that, for example, 4 hours of lectures will be followed by 8 hours of students, with an interlude of one or more weeks allowing students to perform the corresponding 8-9 hours of personal work corresponding to the introductory one hour lecture.

In-person activities may be carried out online if necessary.

 

TYPE

Hours

%

Total

Activities with tuition staff

Lectures

8

8

30 hours

Classrom practice / Seminars / Workshops

16

16

Labs / field / computer-room practicals

0

0

Hospital clinical practice

0

0

Group tutorials

4

4

Placements

0

0

Assessment sessions

2

2

Students personal work

Group work

0

0

70 hours

Individual work

70

70

 

Total

100

 

 

 

 

 

Approximate timetable:

Depending on term schedules, number of students and combined preferences, the work plan may be implemented in a combination of overlapping and/or cyclic modes, as follows: 

[Overlapping mode:]

  • Lectures: One or two hours daily to a total of 8h.
  • Seminars or students presentations: 8-hour blocks at a rate of one to two hours per day.

[Cyclic mode:]

  • Lectures: One or two hours per week to a total of 8h.
  • Seminars or students presentations: One or two weekly sessions of 2 hours, to a total of 16h.

[Common to both ways:]

  • Group tutorials: A total of four hours distributed between the lectures and the seminars.
  • Assessment session: One 2-hour assessment session.

Assessment of students learning:

Continuous assessment of students’ attendance to lectures, seminars, tutoring, and virtual sessions (10%).

Continuous assessment of the participation of students in seminars, their capacity for analysis and discussion of articles or other sources of information, and how they organize and orally present their work (40%).

Written test (50%) consisting of a test with contents similar to those proposed in the seminars, i.e. reasoned resolution of study cases or analysis of scientific or technical publications. The written test shall be performed in the same language as tuition.

Exceptionally, if sanitary conditions require it, non-face-to-face evaluation methods may be included. In this case, students will be informed of the changes made.

In cases with the right to a duly accredited "differentiated evaluation" that entail the impossibility of participating in face-to-face activities, the continuous evaluation may be partially or totally replaced by the performance of non-face-to-face tasks, or by an additional specific test.

 

 

 

 

Resources, bibliography and documentation:

BOOKS:

WEB SITES:

SOFTWARE TOOLS:

JOURNALS:

  • Bioinformatics, BMC Genomics, Genome Biology, Genome Research, Nature Biotechnology, Nucleic Acids Research.
  • Selected articles to be provided on Uniovi Campus Virtual.